The Universal Transport Medium (UTM) was prepared according to the formulations published by the Centers of Disease Control and Prevention (CDC) (4). Hank’s Balanced Salt Solution (HBSS) (Gibco™ Waltham, USA) devoid of the pH-indicator phenol red was implemented as the medium’s isotonic solution. The medium was supplemented with sterile heat-inactivated fetal bovine serum (FBS) (Gibco™ Waltham, USA), gentamicin (Gibco™ Waltham, USA), and amphotericin B (Lonza BioWhittaker™ Walkersville, USA), at final concentrations of 2%, 100μg/ml, and 0.5μg/ml, respectively. Using sterile pipettes, 3 mL of the prepared UTM was dispensed into 15 mL sterile conical centrifuge tubes (Labcon™ Petaluma, USA) and stored at 2-8 °C for one year from the date of production. All UTM production was performed at the Jaber Al-Ahmad Al-Sabah Hospital laboratory, Kuwait.